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. 2014 Aug 22;9(8):e105896. doi: 10.1371/journal.pone.0105896

Figure 1. Major histocompatibility complex (MHC) mismatched mouse model of allogeneic BMT for GvHD induction.

Figure 1

Survival after allogeneic BMT in a C57BL/6 (B6)→BALB/c mouse model (n = 15) of acute GVHD (—♦) (A). Both, syngeneic BALB/c→BALB/c BMT (n = 11) and allogeneic T-cell depleted B6→BALB/c BMT (n = 3) were performed as transplantation controls. Both control groups are represented as dashed lines with a survival rate of 100%. Daily assessed clinical score of allogeneic (including T-cells) vs. syngeneic transplanted mice (B). Weight changes in allogeneic (including T-cells) vs. syngeneic transplanted mice (C). Assessment of engraftment by cellular chimerism analysis 56 days after BMT in the T-cell depleted B6→BALB/c mouse model (D). CD45 positive leukocytes were stained for expression of CD229.1 antigen which is expressed on BALB/c leukocytes but not on B6 cells. Percentages of CD45+ CD229.1+ leukocytes from all splenocytes (SP) or lymph node cells (LN) are shown. Stainings were performed for wild-type B6 (black bars), wild-type BALB/c (white bars) and B6→BALB/c transplanted mice (Tx, grey bars). Chimerism analysis was performed in 3 animals from each group (mean ± SD, ***p<0.001).