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Fluorescent microscopy of GFP production. (a) Effects of PCI on GFP reporter gene transfection with bPEI or PS polyplexes on monolayers of U251 cells. DNA complexed with bPEI, N/P ratio 5:1, DNA concentration 1 μg/ml, PCI light treatment 0.5 J/cm2. b: Percentage transfected cells; number GFP positive/total cell count. [Color figure can be seen in the online version of this article, available at http://wileyonlinelibrary.com/journal/lsm]
