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Modified emulsion PCR conditions for mate pair library
| Stage | Step | Temperature | Time |
|---|---|---|---|
| Hold | Denature | 94°C | 6 minutes |
| Cycle (40) | Denature | 94°C | 30 seconds |
| Anneal | 58°C | 30 seconds | |
| Extend | 72°C | 90 seconds | |
| Cycle (20) | Denature | 94°C | 30 seconds |
| Extend | 58°C | 18 minutes | |
| Hold | 4°C | infinity |
The emulsion PCR conditions of the mate pair library construction for Ion Torrent sequencing of V. cholerae strain R-18308.
