Figure 4.

Predicted miRNAs are developmentally regulated in the hippocampus and may control nervous system development. (a) Venn diagram of miRNA target predictions (TargetScan v. 6.2) for miR-326-3p/330-5p, miR-485-5p, miR-666-3p and miR-761. Less than 15% of predicted targets are shared between any two miRNAs. The numbers in parentheses refer to the number of annotated genes predicted to contain at least one conserved miRNA-binding site. Specific targets are given in the electronic supplementary material, table S2. (b) Venn diagram of GO analysis for miRNA target predictions given in the electronic supplementary material, table S2. The number in parentheses refers to the number of significant GO terms with p-value ≤ 0.01. Eighteen GO terms were found to be enriched for all four miRNAs investigated, showing enrichment for nervous system development and morphogenesis. (c) Predicted miRNA transcripts were developmentally regulated in the hippocampus. The increase in miRNA transcripts from embryonic day 18.5 (E18.5) through postnatal day 21 (P21) (n = 3 animals at each time point) were first normalized to the reference gene U6 snRNA and the abundance expressed relative to E18.5. Fold induction of miRNAs varied from threefold to 12-fold.