. Author manuscript; available in PMC: 2015 Sep 1.

Published in final edited form as: Proteins. 2014 May 6;82(9):2220–2228. doi: 10.1002/prot.24585

Table II.

Solution Characterization of Peptides

Peptide	θ₂₂₂, pH 4.5 (×10³ mdeg/cm² dmol)	θ₂₂₂, pH 7.1 (×10³ mdeg/cm² dmol)	T_M, pH 4.5 (°C)	T_M, pH 7.1 (°C)	ΔT_M^4.5–7.1 (°C)	MW_app (s/D; kDa)^A	MW_exp (trimer) (kDa)
HA2-Stut	−38.1	−21.5	51.4 ± 0.2	35.7 ± 0.1	15.7	14.3	16.4
HA2-Ins	−26.1	−6.7	43.3 ± 0.2	ND^B	ND^B	14.8	16.4
HA2-Rel	−29.0	−18.9	74.5 ± 1.5	~30	~44	14.4	16.4
HA2-Del	−28.2	−9.7	65.5 ± 0.7	17.2 ± 0.7	48.3	16.1	13.8
HA2-Stut-E74D	−29.6	−16.4	37.5 ± 0.2	32.5 ± 0.2 °C	5 °C	15.2	16.3
HA2-Stut-E69D	−22.0	−12.2	42.0 ± 0.4 °C	27.1 ± 0.8 °C	14.9 °C	17.4	16.3

Determined from sedimentation velocity ultracentrifugation at pH 4.5, 20 °C. Full details of the analysis are provided in the Supporting Information. All peptides behaved as a single ideal species.

Accurate T_M at pH 7.1 could not be determined due to a broad thermal transition.