Effect of morphine administration on progesterone (A,B) and allopregnanolone (C,D) levels in the cerebral cortex of C57BL/6J and DBA/2J mice. Morphine (5, 10, and 30 mg/kg, in saline) or saline was administered i.p. 60 minutes before euthanasia. Levels are expressed as nanograms of steroid per gram of tissue and are average ± SEM of values from 11 C57BL/6J mice per group and from 9 DBA/2J mice per group with the exception of the 5 mg/kg group for progesterone levels where n = 7. *p < 0.05, **p < 0.01, and ***p < 0.001 versus the respective saline-treated values; #p < 0.001 versus the respective morphine-treated groups across strains; 2-way ANOVA followed by Bonferroni post hoc test.