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. 2014 Aug 7;2014:310215. doi: 10.1155/2014/310215

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Copyright © 2014 Davide Pascal et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Recombinant ecto-ErbB4-FLAG is expressed and released by cells and is able to interact with soluble NRG1. (a) The correct expression of ecto-ErbB4-FLAG was assayed both in the cell extract and in the conditioned medium (surnatant) of transiently transfected COS7 cells (ecto-ErbB4). Mock samples are COS7 cells transfected with the empty vector. The asterisk indicates an unspecific band. (b) Different amounts of soluble recombinant NRG1β1 tagged with 6 histidine were incubated with conditioned medium of COS7 cells transiently expressing recombinant ecto-ErbB4-FLAG. A FLAG coimmunoprecipitation was performed, followed by Western blot against histidine to recognize NRG1β1. The asterisk indicates the band corresponding to the primary antibody used for coimmunoprecipitation.