Figure 9.

Ecto-ErbB4 stimulates ERK phosphorylation in NOBEC. Western blot analysis of NOBEC cells stimulated with recombinant soluble ecto-ErbB4-FLAG for 0, 5, 10, 15, 30, and 60 min. Western blot was analyzed with antibodies anti-p-AKT and AKT (Panel (a)) and anti-p-ERK 1/2 and ERK (Panel (b)). (c) Bands were analyzed by quantifying the intensity of the pixels per mm² (Image J). The values of the bands corresponding to phosphorylated proteins were normalized to the intensity of the bands corresponding to the total proteins.