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. Author manuscript; available in PMC: 2014 Aug 25.
Published in final edited form as: Anal Chem. 2012 Feb 28;84(6):2761–2768. doi: 10.1021/ac2030893

Figure 1.

Figure 1

A. Affinity purification of anti-Neu5Gc antibodies from IVIG-C over sequential affinity columns with immobilized human- and chimpanzee-serum sialo-glycoproteins.19 B. Generating kappa-enriched or lambda-enriched Fab fragments from affinity-purified anti-Neu5Gc antibodies. Immobilized papain digested purified anti-Neu5Gc antibodies to create two separate Fab fragments and one Fc fragment per antibody molecule. The cleaved fragments were loaded over a Protein A column that binds only the Fc fragments, and the Fab fragments were collected in the run-through. Subsequently, the Fab fragments were loaded over a Protein L column, which binds specifically to kappa light chain, and the lambda-Fab fragments collected in the run-through.