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SDS-PAGE separation of purified human IVIG (IgG) preparations and the specified gel bands (with squares) are cut out for in-gel trypsin digestion. Lane 1&4: MW standards; lane 2: human anti-Neu5Gc antibodies purified by Neu5Gc-affinity chromatography only (Affinity-purified); lane 3: Affinity-purified samples followed by protein A fractionation (Affinity-purified-protein-A).
