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SDS-PAGE separation of Fab and Fc fragments and the specified gel bands (with squares) were cut out for in-gel tryptic digestion. Lane 1: MW standards; lane 2: Lambda-enriched Fab; lane 3: Kappa-enriched Fab; lane 4: Fc. Higher MW bands are aggregates of protein. Band 1*=runthrough from protein L in Figure 1B; band 1^=eluate from protein L in Figure 1B; bands 1–3= eluate from protein A in Figure 1B.
