Figure 1.

Proton-decoupled ¹⁵N solid-state NMR spectra of PS at peptide/lipid ratios of 1.0 mol % in uniaxially oriented lipid bilayers. The spectra are from (3,3,3-²H₃-Ala-11, ¹⁵N-Ala-14)-PS-1 (A and D), (3,3,3-²H₃-Ala-8, ¹⁵N-Leu-15)-PS-2 (B, E, and G), and (3,3,3-²H₃-Ala-11, ¹⁵N-Val-12)-PS-3 (C and F). A–C and G were recorded at sample orientations of the membrane normal parallel to B_o and D–F at alignments of the membrane normal perpendicular to B_o. A–F represent spectra of peptides reconstituted into oriented POPC lipid bilayers, whereas G was obtained in the presence of POPC/POPS (3:1 mol/mole).