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Comparison of the performance of the SNAP-tag fluorescent substrates for use in single-molecule tracking microscopy. The performance is shown in terms of photostability and nonspecific binding. BG-Dy 648 and BG-Dy 649 overlap in the graph. Fluorescent substrates in the lower right corner show little nonspecific attachment to cells, and the most emitted photons per molecule before photobleaching. These substrates are the preferred choice for single-molecule tracking microscopy.
