Figure 4.

Investigation of the effects of invasion inhibitors heparin (Hep) and cytochalasin D (CytD) and the enzyme treatment of chymotrypsin (ChyT) on merozoite-erythrocyte adhesion (WT = wild-type). (A) Portion of merozoites from each egress adhering to erythrocytes after making contact via optical tweezers delivery, <3 min postegress (black) or >3 min postegress (gray). Values are mean ± SE over egress events. (B) Tweezer-driven merozoite detachment force measured from erythrocyte elongation. No significant correlation was observed between typical detachment forces at <3 min postegress and >3 min postegress (provided merozoites are capable of attachment), so values are mean ± SE over total detachment events.