Figure 2.

Phantom experiments. (A) Phantoms with a cylindrical inclusion filled with constant fluorescence and increasing absorptive dye concentrations. The reconstructed absorption appears correctly in the inclusion and is proportional to the absorptive dye concentration (B). (C) The reconstructed fluorescence appears in the inclusion but is suppressed by strong absorption when assuming homogeneous absorption, resulting in a strong negative correlation (D). Usage of the reconstructed heterogeneous absorption avoids this underestimation. Computer simulations using the same phantom shape (E) support the experimental results (F), showing that an absorption map is required to avoid underestimation of fluorescence in absorbing regions.