Figure 5.

Improved fluorescence reconstruction using the shape. (A) The shape of the mouse is derived from the µCT data and used for accurate optical modeling and reconstruction. The mouse shape does not resemble a block which the standalone-FMT assumes for reconstruction. (B, C) The fused FMT reconstruction (left) is compared to the improved reconstruction (right) for transversal slices through liver and tumor, 48 h after injection of the nanopolymer. (B) Absence of the shape information results in erroneous signal in the air (arrow). Usage of the shape for reconstruction avoids this problem. (C) Fusion shows dislocated signal (arrow) above the tumor (green) which is corrected by using the shape for reconstruction. These examples show the importance of the shape information for fluorescence reconstruction.