Skip to main content
. 2014 Aug 13;4(10):1039–1051. doi: 10.7150/thno.7866

Figure 1.

Figure 1

Impact of molecular weight of chitosan on siRNA delivery to the kidney: (a). Delivery using chitosan with various molecular weights. siRNA formulated with chitosan of different molecular weights (N/P=10) were i.v. injected in mice. Kidneys were collected 24 hrs post injection. Four micrograms of RNA isolated from cortex of left and right kidneys was run on 15% denaturing polyacrylamide gels and northern analysis was performed using [γ-32P] ATP labelled siRNA sense strand. Lane 1, 1 ng siRNA duplexes (Ctrl.); Lanes 2-4/5-7/8-10/11-13, 1 ng siRNA/chitosan complex (N/P=10) (Chitosan A, B, C, D, respectively), experiments were performed in groups of two mice (M1, M2) for each formulation. (b). Delivery using chitosan A/siRNA (N/P= 60). siRNA formulated with chitosan A/siRNA (N/P= 60) was injected i.v. in mice (n=2) and kidneys were collected 24 hrs post injection. RNA was obtained from cortex and northern analysis was performed as described above. Lane 1, 1 ng siRNA duplexes (Ctrl.); Lane 2, 1 ng chitosan A/siRNA; Lanes 3-6, RNA from left (L1 and L2) and right kidney (R1 and R2) from mouse 1 (L1 and R1) and mouse 2 (L2 and R2), respectively.