Figure 2.

Specific accumulation of chitosan A/siRNA nanoparticles in kidney PTECs: The kidneys were collected at 24 hrs post-injection from the mice injected with polyplexes formulated with Cy3-siRNA and chitosan A (N/P=60). Sections were made and analysed by fluorescence microscopy, Cy3 signal (red dots) was observed in the kidney (a); the neighbouring sections from the same experiment were labelled with specific antibodies against AQP1 and AQP2 (both in green), clearly demonstrated that chitosan/Cy3-siRNA is co-localised with AQP1 at proximal tubular cells (b), but not with AQP2 in collecting duct cells (c).