Figure 6. On target activity of 15.5-RVD LIC TALENs.
(a) A schematic view of the human STAT6 gene locus with the targeting sites of TALEN pairs T499, T500 and T501 (yellow letters indicate the intronic sequence portion). (b) The TALEN pairs were tested for genome editing activity in HEK 293T cells using the T7EI assay. The panel depicts an agarose gel of one representative result out of two. The white numbers indicate quantified mutation frequencies. *, nonspecific PCR product.