Table 3.

Ovarian follicle populations in female rats dosed with gossypol (25 mg/kg/day) or 0.9% saline (control group) subcutaneously for 15 consecutive days.

Follicles	Control (n = 12)	Gossypol (n = 12)	P value1
Number of normal follicles
Primordial	341.8 ± 25.6	122.6 ± 20.1	<0.0001
Primary	115.5 ± 14.8	49.0 ± 5.03	0.0008635
Secondary	62.6 ± 11.3	20.1 ± 4.54	0.003426
Antral	55.8 ± 6.52	14.5 ± 3.71	<0.0001
Total	575.6 ± 51.8	206.2 ± 27.9	<0.0001
Number of atretic follicles
Primordial	66.3 ± 10.7	168.3 ± 24.1	0.001489
Primary	22.5 ± 2.32	65.8 ± 6.99	<0.0001
Secondary	22.2 ± 4.26	71.7 ± 9.44	0.00023
Antral	8.08 ± 1.52	39.6 ± 3.95	<0.0001
Total	119.1 ± 12.8	345.4 ± 32.8	<0.0001
Proportion of follicles (in %)
Normal	82.1 ± 2.05	36.6 ± 1.73	<0.0001
Atretic	17.8 ± 2.05	63.4 ± 1.73	<0.0001
Number of total follicles
Total	694.7 ± 54.5	551.6 ± 57.2	n.s.

¹Welch's t test.

n.s.: not significant (P > 0.05).