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(A) Schematic diagram of the prism-type total internal fluorescence microscope (TIRFM) setup. (B) Fluorescently labeled and biotinylated-DNA immobilized to a polymer (PEG)-coated surface via biotin–NeutrAvidin interactions. (C) Slide assembly with flow chambers constructed for single molecule imaging. (D) Examples of even and uneven laser illumination of the single molecule surface.
