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. Author manuscript; available in PMC: 2014 Aug 25.
Published in final edited form as: Chem Soc Rev. 2014 Feb 21;43(4):1221–1229. doi: 10.1039/c3cs60201j

Fig. 2.

Fig. 2

(A) cis–trans Isomerization of a Cy3 dye. The pink arrow indicates the rotation with respect to the carbon–carbon double bonds. (B) Schematic of T7 RNA polymerase (RNAP) binding to a duplex DNA. Lifetime and PIFE measurements for DNA alone, with RNAP, and when the DNA bubble is opened. (C) Schematic of the BamHI binding site positioned 1, 7 and 10 bp away from the Cy3 fluorophore. Intensity fold and lifetime fold increases are shown for 1–15 bp distances away from the dye.