Table 1.
List of primers used for detection of mono- and dioxygenase genes.
| Primer | Sequence (5′ to 3′) | Expected size (bp) | Reference |
|---|---|---|---|
| alkBF alkBR |
5′-AACTACMTCGARCAYTACGG-3′ 5′-TGAMGATGTGGTYRCTGTTCC-3′ |
100 | Powell et al. [22] |
| AlkB1F AlkB1R |
5′-TACGGGCACTTCGCGATTGA-3′ 5′-CGCCCAGTTCGAMACGATGTG-3′ |
550 | Kloos et al. [13] |
| nahAc F nahAc R |
5′-TGGCGATGAAGAACTTTTCC-3′ 5′-AACGTACGCTGAACCGAGTC-3′ |
487 | Laurie and Jones [14] |
| PAH-RHD-GPf PAH-RHGPr |
5′-CGG CGC CGA CAA YTT YGT NGG-3′ 5′-GGG GAA CAC GGT GCC RTG DAT RAA-3′ |
292 | Cébron et al. [12] |
| C12OF C12OR |
5′-GCCAACGTCGACGTCTGGCAGCA-3′ 5′-CGCCTTCAAAGTTGATCTGCGTGGTTGGT-3′ |
350 | Sei et al. [23] |
| C23OF C23OR |
5′-AAGAGGCATGGGGGCGCACCGGTTCGA-3′ 5′-TCACCAGCAAACACCTCGTTGCGGTTGCC-3′ |
900 | Sei et al. [23] |