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. 2014 Jun 13;3(6):619–629. doi: 10.1016/j.molmet.2014.05.006

Supplementary Figure 4.

Supplementary Figure 4

Specificity and reversibility of St8sia4 chromatin remodeling upon HFD exposure in MBH. A. Control ChIP with a non-specific isotype IgG antibody. B. Histones H3 and H4 post-translational modifications on the promoter of St8sia4 were analyzed by ChIP. Chromatin extracts were obtained from MBH of mice fed either a standard diet (STD) or a HFD for 3 days (3-day HFD). C. Histones H3 and H4 post-translational modifications on the promoter of St8sia2 were analyzed by ChIP. Chromatin extracts were obtained from MBH of mice fed either a standard diet (STD) or a HFD for 24 h (1-day HFD). D. Association of total histones H3 and H4 and of Pol2-S5P with the promoter of St8sia2 was analyzed by ChIP on MBH extracts obtained after 1, 2 or 3 days of HFD exposure and compared to levels in MBH from mice fed a standard diet (STD). n = 6–8. Data are expressed as mean ± SEM. *p < 0.05.