Figure 6.

Lentiviral-mediated Mof knock-down in the hypothalamus accelerates the onset of DIO. (A) Energy intake of control and MOF KD^MBH mice fed a standard diet, 3 weeks after the injection of the shRNA-containing lentiviral particles. Control: n = 12; MOF KD^MBH: n = 14. (B) Body weight of control and MOF KD^MBH mice fed a standard diet, 3 weeks after the injection of the shRNA-containing lentiviral particles. Control: n = 12; MOF KD^MBH: n = 14. (C)–(H) Effect of Mof knock-down on food intake and body weight during short-term and long-term HFD. Control and MOF KD^MBH mice were fed a HFD for 8 weeks. (C) Analysis of the effect of Mof knock-down on the homeostatic feeding response to HFD. Daily energy intake of control and MOF KD^MBH mice was recorded for 8 days. (D) Cumulative energy intake over 8 days of HFD. (E) Body weight of control and MOF KD^MBH mice fed a HFD for 8 weeks. (F) Body weight gain of control and MOF KD^MBH mice after 8 weeks of HFD feeding. (G) Lean mass and fat mass of control and MOF KD^MBH mice after 8 weeks of HFD. (H) Total energy intake of control and MOF over 8 weeks of HFD feeding. Control: n = 7; MOF KD^MBH: n = 8. Data are expressed as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.