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. Author manuscript; available in PMC: 2014 Sep 26.
Published in final edited form as: Cell. 2013 Sep 26;155(1):188–199. doi: 10.1016/j.cell.2013.09.004

Figure 2. Mitochondria fusion-fission in Agrp neurons varies with metabolic status.

Figure 2

(A) Mitochondria density, and (B) mitochondria coverage in Agrp neurons from fed and 24h food deprived (FD) mice. (C) Cumulative probability distribution of cross-sectional mitochondria area in Agrp neurons of fed and FD mice; insert, relative change in mean mitochondria area between fed and FD mice. (D) Cumulative probability distribution of the aspect ratio (AR) of mitochondria in Agrp neurons; insert, relative changes in mean AR between fed and FD mice. N (fed) = 527 mitochondria/19 cells/4 mice; N (FD) = 729 mitochondria/21 cells/4 mice. (E–H) Differences in mitochondria parameters between mice fed normal chow diet (ND) and a HFD similarly to (A–D). N (ND) = 629 mitochondria/18 cells/3 mice; N (HFD) = 417 mitochondria/18 cells/3 mice. (I) Representative electron micrographs of mitochondria profiles in Agrp neurons from fed and FD mice. (J) Similar to (I), mitochondria labeled in Agrp neurons of mice fed ND or a HFD. (K) Schematic showing the transition from negative energy balance to positive energy balance, and the concomitant changes in mitochondria fusion-fission. All mice were male. Bars represent mean ± SEM. Symbols represent individual values. * P < 0.05, *** P < 0.001. See also Figures S1 and S2.