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. Author manuscript; available in PMC: 2015 Aug 5.
Published in final edited form as: Structure. 2014 Jul 17;22(8):1210–1218. doi: 10.1016/j.str.2014.06.003

Figure 1.

Figure 1

Cryo-EM maps of the ribosome bound with (A) two tRNAs and (B) single tRNA. Large 60S subunit is shown in yellow-orange, small 40S subunit in cyan, E-site tRNA molecule in blue, P-site tRNA molecule in red, hybrid P/E tRNA in purple. (C–D) Molecular structures of the ribosome refined into the maps shown in panels A and B. Colors are as in panels A and B. (E) Fourier shell correlation (FSC) curves for classes representing the 80S•2tRNA and 80S•tRNA complexes (I and II, respectively). FSC calculated using automask3d and FREALIGN for each class were generated as described (Experimental Procedures). For convenience, the X-axis is labeled with spatial frequency Å−1 and with Å. The resolution stated in the text corresponds to a FSC = 0.143 cut-off value shown as a dotted line, for FREALIGN-derived FSC.