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. Author manuscript; available in PMC: 2015 Aug 5.
Published in final edited form as: Structure. 2014 Jul 17;22(8):1210–1218. doi: 10.1016/j.str.2014.06.003

Figure 3.

Figure 3

Differences in the conformations of rotated yeast and bacterial ribosomes bound with P/E tRNA. (A) The tilt of the small 40S subunit relative to the large 60S subunit, resulting in the shift of the head of the small subunit away from the core of the 60S subunit by up to 15 Å. Proteins are not shown for clarity. Comparison of the positions of 18S ribosomal RNA in the rotated 80S ribosome (this work) with E. coli 16S ribosomal RNA (Dunkle et al., 2011) was obtained by structural alignment of 25S rRNA (yellow) and 23S rRNA (not shown). (B) Difference in positions of the tRNA, mRNA and helix 69 between the rotated yeast (this work) and E. coli (Dunkle et al., 2011) ribosomes. The superposition was obtained by structural alignment of large-subunit rRNA of the 80S and 70S complexes, respectively (not shown). (C) Intersubunit rotation axes for yeast (brown) and E. coli (black) ribosomes. The rotation axis for each ribosome was calculated in Chimera (Pettersen et al., 2004), using the small subunit rRNA from non-rotated (bound with two tRNAs) and rotated (bound with single tRNA) ribosome structures. The superposition of yeast and bacterial (Dunkle et al., 2011) ribosome structures was obtained by structural alignment of large-subunit rRNAs. Head and body domains of the small subunit, and the central protuberance (CP) of the large subunit are labeled. 60S is in yellow and orange, 40S in cyan and marine, 70S in grey, 80S rotation axis in brown, 70S rotation axis in black.