Table 1.

Composition of NUT polyacrylamide gel.

Resolving Gel	6 mL (8.2 cm × 9.7 cm × 0.075 cm)	Final Concentration
Urea	2.16 g	6 M
Acrylamide, 60% (60:0.4)	1.5 mL (15%)/1 mL (10%)	15%/10%
MOPS, 0.5 M, pH 7.0	1.2 mL	100 mM
10% (v/v) Triton X-100	0.222 mL	0.37% (v/v)
ddH2O	1.34 mL (15%)/1.84 mL (10%)	N/A
APS, 10% (w/v)	16 µL	0.027% (w/v)
TEMED	8 µL	0.13% (v/v)
Stacking Gel	2 mL (8.2 cm × 9.7 cm × 0.075 cm)	Final Concentration
Urea	0.72 g	6 M
Acrylamide, 60% (60:0.4)	0.3 mL	9%
MOPS, 0.5 M, pH 8.0	0.4 mL	100 mM
10% (v/v) Triton X-100	0.074 mL	0.37% (v/v)
ddH2O	0.646 mL	N/A
APS, 10% (w/v)	12 µL	0.06% (w/v)
TEMED	6 µL	0.3% (v/v)