Table 2.

Composition of TAU polyacrylamide gel.

Resolving Gel	6 mL (8.2 cm × 9.7 cm × 0.075 cm)	Final Concentration
Urea	2.16 g	6 M
Acrylamide, 60% (60:0.4)	1.5 mL	15%
Glacial Acetic Acid	0.3 mL	5% (v/v)
10% (v/v) Triton X-100	0.222 mL	0.37% (v/v)
ddH2O	2.24 mL	N/A
APS, 10% (w/v)	80 µL	0.13% (w/v)
TEMED	40 µL	0.67% (v/v)
Stacking Gel	2 mL (8.2 cm × 9.7 cm × 0.075 cm)	Final Concentration
Urea	0.72 g	6 M
Acrylamide, 60% (60:0.4)	0.3 mL	9%
Glacial Acetic Acid	0.1 mL	5% (v/v)
10% (v/v) Triton X-100	0.074 mL	0.37% (v/v)
ddH2O	0.946 mL	N/A
APS, 10% (w/v)	60 µL	0.3% (w/v)
TEMED	30 µL	1.5% (v/v)