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. Author manuscript; available in PMC: 2014 Aug 25.
Published in final edited form as: Cancer Gene Ther. 2013 Aug 16;20(10):564–575. doi: 10.1038/cgt.2013.53

Figure 4.

Figure 4

Inclusion of Ewing's sarcoma's-activated transcript 2 (EAT-2) adaptor in the vaccine formulation enhances carcinoembryonic antigen (CEA)-specific CD8+ T cells. C57BL/6 (n = 4) mice were co-immunized with equivalent viral particles (VPs) of Ad-CEA mixed with either Ad5- GFP or Ad5-EAT2 (1 × 109 total vps). At 14 dpi, the mice were killed and lymphocytes were isolated from spleen. Multiparameter flow cytometry was used to enumerate the frequency of cytokine-producing CD8+ T cells. Gate were set based on negative control (naive) and placed consistently across samples. The total frequency of CD8+ T cells derived from C57BL/6 mice expressing interferon (IFN)-γ (a), granzyme B (b) or TNFα (c) is shown. The bars represent mean±s.d. Data are representative of two independent experiments with similar results. Statistical analysis was completed using one-way analysis of variance (ANOVA) with a Student–Newman–Keuls post-hoc test, P<0.05 was deemed as a statistically significant difference. * Denotes P<0.05, statistically different from naive animals.