Table 2. Antibiotic stock solutions for use with V. cholerae cultures.

Antibiotic	Solvent	Stock concentration	Working concentration	Storage
Ampicillin	Water	100 mg/ml	100 μg/ml	4°C
Chloramphenicol	Ethanol	34 mg/ml	34 μg/ml	4°C
Kanamycin	Water	45 mg/ml	45 μg/ml	4°C
Polymyxin B	Water	50,000 U/ml	50 U/ ml	4°C
Streptomycin	Water	100 mg/ml	100 μg/ml	4°C
Tetracycline	Methanol	15 mg/ml	15 μg/ml	-20°C

NOTE: All antibiotics should be filter sterilized by passage through a 0.22 μm filter.

NOTE: Dilute antibiotic 1:1,000 into media. For example, when working with a 5 ml broth culture, add 5 μl of the antibiotic stock solution.

NOTE: When making agar plates, autoclave media and let cool to 50°C before adding antibiotics.