Table 1.
Detection of HBV DNA in plasma, liver and peripheral blood mononuclear cells and HBV cccDNA in HBV DNA-reactive liver and lymphoid cell samples in patients on suppressive anti-HBV therapy
| Pre-LT HBV DNA or HBV cccDNA by PCR/NAH (vge/mL or vge/µg total DNA) | |||||
|---|---|---|---|---|---|
| Age/sex/ethnicity | Plasma HBV DNA (detected HBV genes) |
Liver explant HBV DNA (detected HBV genes) |
Liver explant HBV cccDNA |
PBMC HBV DNA (detected HBV genes) |
PBMC HBV cccDNA |
| Case 1 | – | 102–103 (C, S, X, P) | ~103 | – | – |
| 59/M Chinese | |||||
| Case 2 | – | 102–103 (C, S, X, P) | ~103 | – | – |
| 66/M Chinese | |||||
| Case 3 | – | ~104 (C, S, X, P) | <102 | – | – |
| 50/M Chinese | |||||
| Case 4 | – | ~103 (C, S, X, P) | <102 | ~102 (X, P) | – |
| 50/M Polynesian | |||||
| Case 5 | <102 (C, S) | 102–103 (C, S, X, P) | <102 | ~102 (P) | – |
| 60/M Asian | |||||
| Case 6 | ~102 (P) | ~103 (C, S, X, P) | <102 | ~102 (C, S, X, P) | ~102 |
| 67/M Philippine | |||||
| Case 7* | ~102 (C, X, P) | ~103 (C, S, X, P) | ~103 | ~102 (C, S, P) | – |
| 63/F Asian | |||||
| Case 8 | – | 102–103 (C, S, X, P) | ~103 | – | – |
| 55/M Burmese | |||||
| Case 9 | – | 102–103 (C, S, P) | <102 | <102 (P) | – |
| 38/M Chinese | |||||
PCR/NAH, PCR/nucleic acid hybridization assay (sensitivity <10 virus genome equivalents, vge/mL); PBMC, peripheral blood mononuclear cells; HBV cccDNA, covalently closed circular DNA; C, HBV core gene; S, HBV surface gene; P, HBV polymerase gene; X, HBV X gene.
*
Case 7 was HCV antibody positive but HCV RNA was undetectable according to clinical PCR assay.