Skip to main content
. 2014 Aug 15;25(16):2320–2332. doi: 10.1091/mbc.E13-09-0524

FIGURE 5:

FIGURE 5:

Effects of altered TRIII ectodomain shedding on TGF-β–mediated migration and invasion. Stable MDA-MB-231 cell lines were plated in medium that had been preconditioned from the corresponding cell line for 24 h in (A, B) fibronectin-coated Transwell chambers or (C, D) Matrigel-coated Transwell chambers in either the absence (UnT) or presence of 50 pM TGF-β1. Cells were allowed to migrate or invade for 24 h. (A) Representative images of migrated cells. (B) Summary of four experiments. Data normalized to EV UnT and shown as mean ± SEM. Two-way ANOVA for cell line and treatment, p < 0.05. Tukey's multiple-comparisons tests: #p < 0.05 relative to EV UnT; *p < 0.05 relative to EV + TGF-β1; †p < 0.05 relative to ΔS UnT. (C) Representative images of invaded cells. (D) Summary of five experiments. Data normalized to EV UnT and shown as mean ± SEM. Two-way ANOVA for interaction, cell line, and treatment, p < 0.05. Tukey's multiple-comparisons tests: #p < 0.05 relative to EV UnT; *p < 0.05 relative to EV + TGF-β1; †p < 0.05 relative to ΔS UnT.