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. 2014 Aug 15;25(16):2393–2407. doi: 10.1091/mbc.E14-02-0705

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© 2014 Ray et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — FN promotes branching in a LIMK-dependent manner. (A) Treatment of E13 SMG with 0.5 mg/ml exogenous plasma FN (cyan) for 24 h increases F-actin (green) along with an increase in cofilin phosphorylation at Ser-3 (red), as revealed by ICC/confocal imaging. Scale, 20 μm. (B) FN-stimulated branching in E13 epithelial rudiments after 24 h is prevented in the presence of BMS-5, as indicated by bright-field images and morphometric analysis of bud numbers in multiple glands. Scale, 200 μm; n = 4, ***p < 0.001, ANOVA.