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. 2014 Aug 15;25(16):2416–2427. doi: 10.1091/mbc.E14-02-0702

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© 2014 Rao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Syt-1 granules release lumenal contents more quickly than Syt-7 granules. (A) Fusion of a Syt-1-Cherry granule. CgB-pHluorin intensity increases (indicating fusion) at time 0.2 s (within circled region). Syt-1 and CgB disperse quickly after fusion (dotted line). Note loss of fluorescence within circled region (A, B). (C) Syt-7 remains punctate after fusion (bottom). CgB is released slowly. (D) Graphs for C. (E) On average, CgB is more rapidly released from fusing Syt-1 rather than Syt-7 granules. n = 19 for Syt-1 plus CgB; n = 21 for Syt-7 plus CgB. After 2 s, averages ± SEM are significantly (*p < 0.05) different by Student's t test. Scale bar, 960 nm.