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. 2014 Aug 15;25(16):2416–2427. doi: 10.1091/mbc.E14-02-0702

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© 2014 Rao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — A granule membrane protein is released slowly from Syt-7 granules upon fusion. (A) VMAT2-pHluorin fluorescence rapidly disperses from fusing Syt-1 granules (within circled region). (B) Graph for A indicates Syt-1 intensity diminishes after fusion (dotted line), whereas VMAT2 intensity increases and then diminishes. (C, D) VMAT2 movement is restricted from fused Syt-7 granules (D). n = 24 for Syt-1 plus VMAT2; n = 17 for Syt-7 plus VMAT2. Averages ± SEM are significantly different after 1 s (*p < 0.05) by Student's t test. Only negative (–) error bar is shown for VMAT2 plus Syt-7 and positive (+) error bar for VMAT2 plus Syt-1. Scale bar, 960 nm.