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. 2014 Aug 15;25(16):2428–2443. doi: 10.1091/mbc.E14-02-0710

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© 2014 Pantazopoulou et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — MyoE-dependent and -independent functions of F-actin. (A) Latrunculin B treatment delocalizes GFP-RabE–containing membranes at the SPK to the MT conveyor belt. Derived from Supplemental Movie S8. (B) An example of a myoE∆ tip in which the GFP-RabE crescent is thicker in a slightly subapical position. (C) Kymograph covering the position of a cortical MT, showing that it serves as track for RabE^RAB11 carriers moving in both directions and the uniform speeds characteristic of MT-dependent movements. (D) Maximal-intensity projection of a middle-plane time stack showing how moving GFP-RabE carriers delineate trajectories of MTs. (E) Micrographs from time stacks of images and the corresponding kymographs, showing effects of F-actin and MT depolymerization on the tip-and-apical crescent-associated GFP-RabE carriers. Both drugs delocalized these carriers from the tips of myoE∆ cells, but these carriers moved only when MTs were present. (F) Isolated frame from a time stack (Supplemental Movie S11) showing microfilament-like material decorated with GFP-RabE carriers. A hyphal tip in which actin cables are decorated with GFP-tropomyosin is shown for comparison. Experiments were carried out with strain MAD4120 and its myoE∆ derivative, MAD4409. The TpmA-GFP inset corresponds to MAD1750.