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. 2014 Aug 15;25(16):2428–2443. doi: 10.1091/mbc.E14-02-0710

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© 2014 Pantazopoulou et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Latrunculin B displaces RabE^RAB11 carriers to MTs. (A) Maximal-intensity projection of middle-plane time stack showing trajectories of mCherry-RabE and MyoE-GFP. The finding that latB displaced carriers from the SPK to the MT conveyor belt allowed us to determine that the trajectories of MyoE and RabE colocalize, delineating MTs. (B) Kymograph of the latrunculin B–treated cell, showing how MyoE-GFP and mCherry-RabE colocalize on moving structures. These experiments were carried out with strain MAD4403 cultured on fructose, with MyoE-GFP being expressed from an endogenously tagged myoE allele (Taheri-Talesh et al., 2012).