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. 2014 Aug 15;25(16):2472–2484. doi: 10.1091/mbc.E14-04-0865

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© 2014 Buchwalter et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Within the nucleus, Nup50 overlaps with euchromatin and segregates from heterochromatin. (A) SIM of C2C12 cells stably expressing 2xGFP-Nup50 and costained for H3K4me3 and DNA (DRAQ5). (B) SIM of C2C12 cells stably expressing 2xGFP-Nup50 and costained for H3K9me3 and DNA (DRAQ5) in the absence (top) or presence (bottom two) of 5 μg/ml Act D. Arrowheads indicate heterochromatic foci.