FIGURE 8:

Depletion of Nup50 does not affect cell proliferation but limits differentiation. (A) Comparison of cell number over several days of growth of C2C12 cells (black trace) or C2C12 cells stably expressing a Nup50 shRNA (orange trace) that were initially seeded at equal density. (B) Western blot of Nup50 in C2C12 cells stably transduced with pLKO.1 empty vector or two different Nup50 shRNAs. (C) MHC staining of differentiated myotubes in C2C12 cells stably transduced with pLKO.1 empty vector or two different Nup50 shRNAs after 72 h in differentiation medium. (D) Quantification of number of nuclei per myotube observed in C2C12 cells expressing pLKO.1 empty vector or two different Nup50 shRNAs after 72 h in differentiation medium as shown in C. N > 189 cells/condition. *p <0.05 by t test with Welch's correction. (E) Fusion index (percentage of nuclei in myotubes containing n > 2 nuclei/total nuclei in field) for C2C12 cells expressing pLKO.1 empty vector or two different Nup50 shRNAs after 72 h in differentiation medium as shown in C. N > 2000 cells/condition. *p < 0.05 by t test.