. Author manuscript; available in PMC: 2014 Aug 25.

Published in final edited form as: J Chromatogr A. 2006 Sep 27;1135(1):27–35. doi: 10.1016/j.chroma.2006.09.015

Table 4.

Accuracy of the HPLC method for determination of ten saponins

Compound^a	Original (μg)	Spiked (μg)	Observed (μg)	Recovery (%)	Mean (%)	RSD (%)
1	732	700	1412	97.0	99.1	2.5
		500	1224	98.3
		300	1038	102.0
2	1075	1000	2081	100.6	98.7	1.9
		700	1752	96.7
		500	1568	98.6
3	139	100	236	97.1	97.5	1.2
		70	208	98.7
		50	187	96.4
4	417	400	819	100.6	99.5	1.4
		300	717	100.1
		200	613	97.9
5	736	700	1451	102.1	100.2	1.6
		500	1232	99.2
		300	1034	99.2
6	2425	2000	4411	99.3	101.1	1.5
		1500	3958	102.2
		1000	3442	101.7
7	2099	2000	4082	99.1	99.0	0.6
		1500	3592	99.5
		1000	3082	98.3
8	284	300	580	98.5	98.5	0.3
		200	481	98.9
		100	380	98.3
9	597	500	1079	96.5	96.8	0.6
		300	889	97.5
		200	790	96.4
10	296	300	582	95.3	95.9	1.2
		200	487	95.1
		100	394	97.2

Recovery (%) = (Observed amount − original amount)/spiked amount × 100%, RSD (%) = (SD/mean) × 100%.

(1) deapi-platycoside E; (2) platycoside E; (3) deapi-platycodin D₃; (4) platycodin D₃; (5) deapi-platycodin D; (6) platycodin D; (7) polygalacin D; (8) 3″-O-acetyl polygalacin D; (9) platycodin A; and (10) 2″-O-acetyl polygalacin D.