Figure 6. Gsk3α deletion promotes E2F-1 recruitment in vivo.
(A) Representative immunoblots show phospho-Retinoblastoma protein (pRb) (Ser790) and E2F-1 from 3 wk post-MI LV lysates. (B) Quantification shows comparable pRb and significantly increased levels of E2F-1 in the post-MI KO hearts, n=4-5. Results are expressed as mean±SEM, ** P<0.01. (C) Co-immunoprecipitation using an antibody against E2F-1 followed by immunoblotting for GSK-3α in neonatal rat ventricular myocytes (NRVM) confirms a physical interaction between these molecules.
