Figure 4.

Virus replication and gene expression during acute infection of mice. (A) Virus replication in the eye. Each point represents the geometric mean titer of at least 15 eye swabs. (B) Virus replication in the TG. Each point represents the geometric mean titer of at least 10 TG. There are no statistically significant differences between the titers of WT and M138 viruses in panels (A) and (B). (C) Viral genome and transcript levels in TG at 7 dpi. Each point represents a value from one TG. Virus names are shown at the bottom, and the molecules assayed are labeled at the top of each graph. Vertical axes show logarithmic values, and RNA levels were normalized to viral genome levels. The horizontal lines represent the geometric means (any undetectable values were included in the calculations of means as logs of the detection limits). P values for the two viruses compared are shown on top of the brackets. (D) Correlations between transcript levels in TG infected by the viruses indicated at the top of each plot at 7 dpi. The level of gC transcripts or LATs were plotted against the level of ICP0 transcripts in the same TG. One TG that exhibited undetectable levels of gC and ICP0 transcripts was excluded from the plots. R² and P values for each correlation are shown at the upper left corners of the plots. See also Figure S3 and Table S1.