Fig. 2.

RPTEC/TERT1 cells respond to 24 h Cd exposure by upregulating HMOX1 and NQO1 but not GCLC. After 24 h of treatment with Cd at various concentrations, RPTEC/TERT1 cells showed no change in (A) GCLC at any concentration. There was a significant increase in gene expression at the highest concentration, 10 μM Cd, of (B) HMOX1 and (C) NQO1. Bars represent mean fold expression (n = 3) ± SEM. All genes of interest were normalized to ACTB. 0 μM, where denoted, was set as 1. *Significant difference from 0 μM Cd, p < 0.01, ^#Significant difference from 10 μM Cd, p < 0.01, and ⁺Significant difference from 0 μM Cd, p < 0.05.