Table 3.
Agreement between model and map.
Model | EM map (hand) | Coordinatesa | Correlation |
---|---|---|---|
1 | Virion (+) | X-ray | 0.93 |
2 | Virion (–) | X-ray | 0.86 |
3 | Procapsid (+) | X-ray | 0.58 |
4 | Procapsid (–) | X-ray | 0.31 |
5 | Procapsid (+) | F+, Grot | 0.93 |
6 | Procapsid (+) | F+, Gunrot | 0.93 |
7 | Procapsid (–) | F+, Grot | 0.74 |
8 | Procapsid (–) | F–, Grot | 0.91 |
9 | Procapsid (–) | F–, Gunrot | 0.91 |
10 | Provirion (+) | F+, Grot | 0.88 |
11 | Provirion (+) | F+, Gunrot | 0.90 |
12b | Procapsid (+) | Procapsid (–) | 0.69 |
13 | Virion (+) | Virion (–) | 0.95 |
X-ray refers to the X-ray crystallographic coordinates for the whole of the F and C proteins. F+ refers to the truncated model of the F protein as fitted into the procapsid (+) map. F– refers to the truncated model of the F protein as fitted into the procapsid (–) map. Gunrot refers to the truncated model of the G protein in the same orientation as found in the virion. Grot refers to the truncated model of the G protein rotated by 20° from the virion orientation.
Correlation between maps of opposite hand. This demonstrates that the procapsid map has greater ‘handedness’ than that of the virion.