Fig. 5.

Hepatic G6Pase activity and plasma metabolic parameters in LS-G6pc^−/− mice. Three to five mice were analyzed for each group of LS-G6pc^−/− mice. G6P phosphohydrolase activity in the liver microsomes (A), and blood glucose (B), total cholesterol (C), triglyceride (D), ALT (E) and AST (F) levels of LS-G6pc^−/− mice were determined at the indicated ages in weeks (w) or months (m). Four age-matched wild-type mice were analyzed for each group. Because of the similarities of the respective metabolites in each group of control mice, results shown are pooled data of age 2 weeks-20 months. (A′) Liver lysates were subjected to western blotting and G6Pase-α expression was visualized with anti-G6Pase-α antibody. (B) Glucose levels were determined in the fed state (dark gray bars) or upon 6 hours of fasting (light gray bars). Data are presented as mean ± s.d. Values that are significantly different from control mice (P<0.05*, P<0.01** and ***P<0.005) are indicated.