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. 2014 Jul 4;7(9):1111–1122. doi: 10.1242/dmm.015453

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Granulomas in adult zebrafish after intraperitoneal infection. (A) Coronal section of adult zebrafish at 50 days after infection with M. marinum E11. Multifocal to coalescing granulomas (black arrows) affecting the meninges and submeningeal space structure can be seen, the brain parenchymal tissue (*) beneath the granulomas shows minimal lymphocytic inflammation, and the regional meningeal blood vessels are markedly congested (yellow arrow). Scale bar: 100 μm. Granuloma is enlarged in panels B and C. (B) Granulomas are composed of epithelioid and foamy macrophages (black arrow) that occasionally exhibited degeneration and necrosis (arrowheads). Scale bar: 20 μm. (C) Same granuloma as shown in panel B stained with ZN. Multiple acid-fast bacilli are present in the cytoplasm of the macrophages (black arrows). Scale bar: 20 μm.