Figure 3.
Expression of cytokines, chemokines, and growth factors in BJ cells, under homeostatic and inflammatory conditions. Cells were plated at 8 × 105 cells/dish in 60 mm dishes in a final volume of 6 mL of medium and treated with 1 μg of LPS during 6 h (LPS), or left untreated (Ctrl), at 37°C, with 5% CO2. Total RNA was isolated and retrotranscribed as indicated in Section 2. The mRNA levels were assessed by quantitative real-time RT-PCR. Gene expression is indicated as genes studied/10 000 molecules of the reference gene HPRT1. Values represent the mean ± SD from four independent experiments. Kruskall-Wallis test followed by Dunn's posttest statistical analysis was performed between cytokines, chemokines, and growth factor expression under homeostatic and inflammatory conditions (**P < 0.01; ***P < 0.001) and among cytokines, chemokines, and growth factor expression under homeostatic conditions (## < 0.01; ### < 0.001). IL: interleukin; EGF: epidermal growth factor.