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Reaction system for real-time fluorescence quantitative PCR.
| Components | Sample amount |
|---|---|
| 10 × buffer (without Mg2+) | 3 μL |
| MgCl2 (25 mM) | 3 μL |
| dNTP (25 mM) | 0.36 μL |
| Sense prime (10 μM) | 1 μL |
| Antisense primer (10 μM) | 1 μL |
| TaqMan probe (10 μM) | 0.6 μL |
| Taq enzyme (5 U/μL) | 0.3 μL |
| Double distilled water | 18.74 μL |
| Template DNA | 2 μL |
